Sunday 28 April 2013

The Gear I use for UV-photography


I will keep this article quite brief, because all the fundamental technical aspects of the UV-photography that I currently apply, have been established and published by others already. So I’m not going to restate the fundamentals.
Instead I’ll give you two links as starting points:


These sites are pretty comprehensive and should include links to almost everything that I have found on the web, so far. Obviously, you can also type the words “UV-photography”, “UVVIS”, “UV-flowers” or any others in a search engine and see what you get. I predict that most of the hits will bring up sites that are already listed on the resources sited above.

Currently, I use the following gear:

Camera: Panasonic Lumix G1 

I had this camera modified by a professional service:
It has been equipped with the UVI filter that allows a transmission of wavelengths from 350 – 1100 nm. So, according to this specification the range from roughly 350-400 nm is available for UV-photography.  

Lensens:

  1. EL-Nikkor 80 mm /f5.6 (enlarger lens): After I had severe trouble with a newer model, I purchased the old, all-metal version, which works fine for macro work. However, since it is an enlarger lens, it needs to be mounted on a focusing helicoid (what I did) or alternatively a bellow. Last but not least, it is challenging to find a step-ring that fits into the very unusual filter thread (34.5 mm). So you have to be a bit creative until everything fits together. My construction is only good for macro-work, since it doesn’t focus to infinity. On the other hand I have found some extension tubes on Ebay that allow a magnification of roughly 2:1. This lens is mentioned on several websites as a good UV lens.
  2. Novoflex Noflexar 35 mm /f 3.5: This is another frequently mentioned UV-capable lens. It can be used for macro work and focuses to infinity. I use it mainly if I want to capture landscapes or other objects that are too big for the above lens.


Filters:

  1. Optic Makkario IR-Neutralisationfilter NG, for photos in the visible spectrum 400-700 nm.
  2. Baader-U (Venus-filter) 2”, for UV-images. These days this seems to be only single filter that transmits UV-light without relevant IR contamination. It is also recommended on many websites. Personally, I started with a filter stack (UV-transmission and IR blocking) that didn’t provide really satisfying results. Only the blue-channel was quite clean.
My light-source is the sun. However, I’m considering purchasing a flash with UV emission.
I always mount the camera on a tripod (Gitzo-Explorer, GT2540EX) with a Markins ball-head, and I use a cable release. 



Tussilago farfara, commonly known as Coltsfoot: Noflexar 35/f3.5 at f8, 4 sec. ISO 400
Since it was windy, this image suffers from some motion-blur.

Saturday 27 April 2013

Introduction

About a year ago I started taking UV-photos, mostly of flowers. I was fascinated by the UV-patterns of blossoms that are invisible to the human eye. However, these patterns are well known in literature at least since the 1970s. Additionally, there are many flowers that don't show any significant UV-reflection. 
Kevan et al. (2001) have pointed out, that UV-light contributes to the visual orientation of many species, including bees and other insects, but the importance is not fundamentally different from other wavelengths, e.g. blue and green light [1]. 

Bees and many other insects have three colour receptors in their eyes, similar to humans. However, the difference is that they do have a receptor sensitive to UV, while they are less sensitive to longer wavelengths that we see as red. In summary, their visible spectrum is shifted towards the shorter wavelengths (compared to ours). 

I’ve been thinking for a while how to best visualise the colours that bees see. It seems logical to me to combine the entire spectrum that their eyes can detect, which means near UV (~340-380 nm), blue (max. 440 nm) and green (max. 540 nm). Since we cannot see UV, I’ve “pushed” these wavelengths into the RGB colour space. So UV becomes blue, blue becomes green and green becomes red.

I’m planning to illustrate and explain this approach in more detail, but for now I just want to show some flowers in “human colours”, the UV image, and the simulated “bee colour”:


Let’s start with Common Daisy (Bellis perennis) that for our eyes has white petals and yellow pollen (that doesn’t reflect blue light):



The UV-image shows that the petals do reflect part of the UV-rays, although not as strongly as visible light. The pollen doesn’t really reflect UV-rays and is therefore UV-dark:



Now, let’s remove the original red channel and combine UV with blue and green and see the result:






The Lesser celandine (Ranunculus ficaria) is an example of a yellow blossom that seems more or less evenly yellow for our eyes (a bit darker in the middle):
 



However, it has a strong UV pattern, since the outer petals show a strong UV reflection, while the middle of the blossom doesn’t:



And here is the image if I push UV, blue and green into the RGB colour space. This bright bee-pink is the result of the UV (bee-blue) and green (bee-red) reflection:





As a last example for today let’s have a look at the blue flowers of the Grape Hyacinth (Muscari sp.). That’s how they appear to our eyes:


The UV-image shows that the flower does reflect some UV light:




The simulated bee-colour is a mixture of UV (bee-blue) with blue (bee-green) which results in bee-turquoise, while the entrance of the flower, that we see as white, turns into bee-yellow:
 




What I see when I browse through my images and compare the simulated bee-colours with the image in the human colour-spectrum is, that the bee-coloured flowers stand out more against the background. This makes sense for insect-pollinated plants, since they have undergone a co-evolution with their pollinators.



The primary goal of this blog is to get in touch with people with interest in this very special topic and to start a scientific and constructive discussion. So far, I haven’t found simulated bee-colours that look similar to mine. Therefore, I’m also very interested in hints about related work.


Literature:
[1] Peter G. Kevan, Lars Chittka and Adrian G. Dyer: Limits to the Salience of Ultraviolet: Lessons from Colour Vision in Bees and Birds. The Journal of Experimental Biology 204, 2571–2580 (2001).